Expression of differentiation markers by chick embryo neuroretinal cells in vivo and in culture.

نویسندگان

  • D I de Pomerai
  • B Kotecha
  • M Flor-Henry
  • C Fullick
  • A Young
  • M A Gali
چکیده

Several markers of chick neuroretinal differentiation were monitored in vivo and in culture. All increase markedly between 7 and 20 days of embryonic development in vivo. In vitro, endogenous GABA levels decrease almost immediately, while other neuronal markers increase as in vivo for 2 to 5 days before declining (choline acetyltransferase, acetyl cholinesterase, glutamic acid decarboxylase). Neuronal cell surface markers (binding sites for tetanus toxin, alpha-bungarotoxin, muscimol), however, reach maximal levels only after 8 days in vitro. Glial markers such as carbonic anhydrase and hydrocortisone-induced glutamine synthetase activities are also expressed only transiently in culture.

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عنوان ژورنال:
  • Journal of embryology and experimental morphology

دوره 77  شماره 

صفحات  -

تاریخ انتشار 1983